elute
Definition
Verb: To separate or wash out (a substance) from a material by passing a solvent through it. This process is particularly fundamental in chromatography, where a mobile phase (the solvent) moves through a stationary phase, carrying different components of a mixture at different rates to separate them.
Usage
The verb "elute" describes the specific action in a separation process where a solvent causes adsorbed substances to move from a solid medium. It is primarily used in scientific contexts, especially chemistry and biochemistry.
Examples
- Verb:
- The goal is to elute the protein from the column using a buffer with a high salt concentration.
- In this experiment, we will elute the compounds with a gradient of increasing solvent polarity.
- The desired component was successfully eluted after ten minutes.
Advanced Usage
- "to elute something from something": This is the standard construction, specifying both the substance being removed and the medium it is removed from.
- The researcher needed to elute the antibiotic from the resin for further testing.
Variants and Related Words
- Elution (n): The process or result of eluting.
- The elution profile showed two distinct peaks.
- Eluent (n): The solvent or solution used to carry out the elution.
- The chosen eluent was a mixture of water and methanol.
Synonyms
- Wash out: To remove by the action of a liquid (less technical).
- Extract: To remove a substance, often implying a general removal rather than the specific chromatographic process.
Antonyms
- Adsorb: To accumulate (a substance) on a surface in a condensed layer.
- Bind: To become attached or fixed to a surface.
Verb
-
wash out with a solvent, as in chromatography
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