safranin

/'sæfrənin/
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safranin

A scientist stains a tissue sample with safranin for microscopic examination.

Definition
  1. Noun:
    • A type of synthetic dye: Safranin refers to any of a group of primarily red or pink dyes used in biological staining and industrial applications.
    • A chemical compound: It is a cationic dye belonging to the azine class, commonly used to stain cell nuclei, cartilage, and mucin.
Examples of Usage
  • Noun:
    • The technician used safranin as a counterstain in the Gram staining procedure.
    • Under the microscope, the nuclei were clearly visible because they absorbed the safranin.
Advanced Usage
  • "Safranin O": This is the most common and specific form of safranin dye used in histology and microbiology.
    • Safranin O is applied last in the Gram stain to color Gram-negative bacteria pink.
Variants and Related Words
  • Safranine: An alternative spelling for safranin.
  • Counterstain: A secondary stain applied to provide contrast, a role often filled by safranin in staining protocols.
Synonyms
  • Basic red 2: One of its standard chemical names.
  • Cationic dye: Describes its chemical property of having a positive charge, which is why it binds to negatively charged cellular components.
Related Phrases and Terms
  • Gram staining: A fundamental microbiological technique where safranin is a key component.
    • After decolorization, the slide is flooded with safranin for one minute.
  • Nuclear stain: A stain that colors the cell nucleus; safranin can function as one.
    • Safranin and methyl green are both used as nuclear stains in plant histology.
safranin

A scientist stains a tissue sample with safranin for microscopic examination.

Noun
  1. any of a class of chiefly red organic dyes

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