cosmid
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Definition
Noun: 1. (Genetics) A type of large cloning vector: A cosmid is a laboratory-constructed DNA molecule, derived from a bacteriophage (a virus that infects bacteria), that is used to clone (make identical copies of) relatively large fragments of foreign DNA, such as genes or gene fragments, inside a bacterial host.
Examples of Usage
- Noun:
- The researchers inserted the human gene fragment into a cosmid for amplification.
- This cosmid library contains large segments of the plant's genome.
- Compared to plasmids, cosmids can carry much larger pieces of DNA.
Advanced Usage
- Cosmid cloning: The process of using a cosmid as a vector to replicate a DNA insert.
- Cosmid cloning was essential for constructing physical maps of complex genomes.
- Cosmid library: A collection of cloned DNA fragments, each carried within a cosmid vector, which together represent the entire genome of an organism.
- Scientists screened the cosmid library to find the sequence responsible for the trait.
Variants and Related Words
- Vector (n): In molecular biology, a DNA molecule (such as a plasmid, virus, or cosmid) used as a vehicle to artificially carry foreign genetic material into a host cell.
- Bacteriophage (n): A virus that infects and replicates within bacteria. Cosmids utilize specific sequences (cos sites) from bacteriophages.
- Plasmid (n): A smaller, circular DNA molecule, distinct from a cell's chromosomal DNA, often used as a cloning vector for smaller DNA fragments.
Synonyms
- Cloning vector: A general term for any DNA molecule used to carry foreign DNA into a host cell for replication.
Related Terms and Concepts
- Cos sites: Cohesive end sites; specific DNA sequences from bacteriophage lambda that are present in cosmids and allow the vector DNA to be packaged into phage particles.
- Genomic library: A collection of clones that contains fragments representing the entire genome of an organism; a cosmid library is one type of genomic library.
- Recombinant DNA technology: The set of techniques used to join together DNA molecules from different sources; cosmids are a tool in this field.
Noun
- (genetics) a large vector that is made from a bacteriophage and used to clone genes or gene fragments