heterophil test

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heterophil test

A laboratory technician performs a heterophil test on a blood sample.

Definition

Noun: A diagnostic blood test that detects the presence of heterophil antibodies, which are antibodies that react with antigens from a different species. In this specific test, a positive reaction, indicated by the agglutination (clumping) of sheep red blood cells, is used as an indicator for infectious mononucleosis.

Usage

The heterophil test is primarily used in medical diagnostics. * It is ordered when a patient presents symptoms suggestive of infectious mononucleosis, such as fever, sore throat, swollen lymph nodes, and fatigue. * The test is performed on a sample of the patient's blood serum. * A laboratory technician observes if the serum causes sheep red blood cells to agglutinate. Visible clumping is a positive result.

Examples
  • The doctor ordered a heterophil test to confirm her suspicion of mono.
  • A positive heterophil test, along with the patient's clinical symptoms, is diagnostic for infectious mononucleosis.
  • The heterophil test is also known as the Monospot test, which is a rapid commercial version of it.
Advanced Usage
  • The test is most accurate when performed during the second to fourth week of illness, as heterophil antibody levels may not be detectable early in the infection.
  • A negative heterophil test does not completely rule out infectious mononucleosis, especially in children, as they may produce fewer heterophil antibodies. In such cases, tests for Epstein-Barr virus-specific antibodies may be required.
Variants and Related Words
  • Monospot test: A common, rapid slide agglutination test that is a specific type of heterophil test used for diagnosing infectious mononucleosis.
  • Paul-Bunnell test: The original, classic heterophil antibody test upon which modern versions like the Monospot are based.
Synonyms
  • Monospot test (a specific brand/type)
  • Heterophil antibody test
  • Infectious mononucleosis test
Notes on Meaning

The term specifically refers to the medical laboratory procedure. The core concept is the detection of heterophil antibodies, which are a type of immunoglobulin produced in response to Epstein-Barr virus infection but which cross-react with antigens on sheep, horse, or bovine red blood cells.

heterophil test

A laboratory technician performs a heterophil test on a blood sample.

Noun
  1. a blood test to detect heterophil antibodies that agglutinate sheep red blood cells; positive result indicates infectious mononucleosis