immunohistochemistry
A scientist examines a tissue slide under a microscope using immunohistochemistry.
Noun: A laboratory technique used to visualize and locate specific proteins (antigens) in tissue samples. It works by using antibodies that bind to the target protein; these antibodies are linked to markers, such as fluorescent dyes or enzymes, which produce a detectable color or glow, allowing scientists to see where the protein is present under a microscope.
Immunohistochemistry is a fundamental tool in biomedical research and diagnostic pathology. * The diagnosis was confirmed using immunohistochemistry, which revealed the presence of the tumor-specific marker. * Immunohistochemistry on the brain tissue sample showed abnormal protein aggregates characteristic of the disease.* * Researchers employed immunohistochemistry to map the distribution of the receptor in different organs.*
- "Immunohistochemical" (adjective): Describes something related to or obtained through immunohistochemistry.
- The immunohistochemical staining pattern was consistent with melanoma.
- They performed an immunohistochemical analysis of the biopsy.
- "Immunohistochemically" (adverb): Describes an action performed using this technique.
- The protein was immunohistochemically localized to the cell membrane.
- Immunohistochemical (adj): Pertaining to immunohistochemistry.
- Immunohistochemically (adv): In a manner relating to immunohistochemistry.
- Immunocytochemistry (n): A closely related technique used to localize specific antigens in cultured cells rather than tissue sections.
- Immunofluorescence (n): A specific type of immunohistochemistry that uses fluorescent dyes as markers.
- Immunostaining (a more general term often used interchangeably in context)
- Immunohistochemical staining
- Antibody-based tissue staining
- Antibody-antigen interaction: The foundational principle on which immunohistochemistry is based.
- Chromogenic detection: A common method where an enzyme-linked antibody produces a colored precipitate.
- Fluorescent detection: A method where a fluorescent dye-linked antibody emits light of a specific color when excited by a particular wavelength.
- Formalin-fixed, paraffin-embedded (FFPE) tissue: The most common type of tissue sample prepared for immunohistochemistry.
A scientist examines a tissue slide under a microscope using immunohistochemistry.
- an assay that shows specific antigens in tissues by the use of markers that are either fluorescent dyes or enzymes (such as horseradish peroxidase)