elisa

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elisa

A scientist performs an ELISA test in the laboratory.

Definition
  1. Noun:
    • Enzyme-Linked Immunosorbent Assay: A laboratory technique used to detect and measure specific substances, such as proteins, antibodies, hormones, or pathogens (like viruses and bacteria), in a liquid sample. The assay relies on an enzymatic reaction that produces a detectable color change to indicate the presence and quantity of the target substance.
Examples of Usage
  • Noun:
    • The doctor ordered an ELISA to test for the presence of antibodies against the virus.
    • ELISA is a common method for screening blood donations for infectious diseases.
    • Researchers used an ELISA kit to quantify the concentration of the protein in their samples.
Advanced Usage
  • "to run an ELISA": to perform the ELISA test procedure.
    • The lab technician will run an ELISA on the serum samples tomorrow.
  • "ELISA test/assay/kit": common collocations specifying the type of test or the commercial product used.
    • The results of the ELISA test were confirmed by a more specific method.
    • We purchased a commercial ELISA kit for faster analysis.
Variants and Related Words
  • Enzyme immunoassay (EIA) (n): A broader category of assays that ELISA belongs to, which use enzyme-labeled molecules for detection.
  • Immunoassay (n): A general term for any biochemical test that measures the concentration of a substance using the specific binding of an antibody to its antigen.
Synonyms
  • Enzyme immunoassay: A very close synonym, though "ELISA" often refers to a specific plate-based format of an EIA.
Related Phrases
  • Direct ELISA: A format where the antigen is immobilized and detected directly by an enzyme-linked antibody.
  • Indirect ELISA: A format often used for antibody detection, where the primary antibody is detected by an enzyme-linked secondary antibody.
  • Sandwich ELISA: A highly specific format used to detect antigens, where the antigen is "sandwiched" between a capture antibody and a detection antibody.
  • Competitive ELISA: A format used to measure small antigens, where the sample antigen competes with a labeled antigen for binding sites.
elisa

A scientist performs an ELISA test in the laboratory.

Noun
  1. an assay that relies on an enzymatic conversion reaction and is used to detect the presence of specific substances (such as enzymes or viruses or antibodies or bacteria)